I.System Description

Biological (cells, mitochondria, etc.) measurement of oxygen characteristics of computer analysis system, the project the Department of Chinese People's Liberation Army 304 Hospital and the joint development.

II.System structure and working principle

1. Blood pressure
According to physics, to a certain temperature conditions, the gas solubility in liquids, in a certain range it is proportional to the partial pressure, that is, the higher its partial pressure, was dissolved more the less the other hand, it blood oxygen content of different machines, the partial pressure of oxygen is different, high blood pressure, blood oxygen content, and low blood pressure, blood oxygen content of less, it directly reflects the blood partial pressure of oxygen in blood how much oxygen.
Clinical detection of blood oxygen partial pressure of the methods, namely, arterial blood samples for blood gas analysis, and the other is non-invasive transcutaneous measurement of blood pressure.

2. Oxygen saturation
Red blood cells in the blood plays a role of oxygen and hemoglobin to be completed by. Hemoglobin molecule in hemoglobin with four oxygen binding in the standard mode, if the hemoglobin with oxygen exposure, when reaching saturation, the per gram of hemoglobin can be combined with 1. 34mL oxygen when the oxygen saturation of hemoglobin of 100 %, while in fact most of oxygen saturation in non-saturation, normal for 95-98%. Oxygen saturation of blood oxygen saturation can be used to measure equipment.
Oxygen saturation of hemoglobin is only a reflection of the extent of combining with oxygen, can only indirectly reflect the amount of oxygen, rather than the direct representative of blood oxygen content.

3. Blood pressure and oxygen saturation relations
Between the two is not equivalent to, but the relationship between a fixed, usually referred to as "S" curve, that is, oxyhemoglobin dissociation curve. Under normal circumstances, when the partial pressure of oxygen to 100mmHg, the corresponding oxygen saturation was 98%, partial pressure of oxygen fell to 60mmHg, the oxygen saturation dropped to 90%, that is, a decrease of oxygen partial pressure 40mmHg, oxygen saturation decreased by only 8%, only when the partial pressure of oxygen less than 40mmHg, the oxygen saturation will decrease rapidly. It is only based on oxygen saturation to determine the hypoxic conditions, while the body is often neglected early hypoxia, was to conceal, easy to delayed diagnosis or failure to give timely treatment.

4. Under conditions of high oxygen partial pressure monitoring
When atmospheric oxygen or liquid oxygen at the high-drip, blood pressure often is greater than 100mmHg, blood oxygen saturation reached saturation levels, that is 100%, partial pressure of oxygen and then how changes in oxygen saturation is not reflected by the instrument , and transcutaneous oxygen pressure measurement can fully demonstrate its clinical value.

Instructions:
1. Function: Characteristics of biological materials is the evaluation of oxygen vitality and function of organisms an important indicator, so in biology, low temperature biology, physiology, pathology, genetics, clinical medicine, pharmacology, nutrition and other fields, how to its measurement and analysis, has attracted much public concern and the need to resolve an important issue.
Mitochondria is an important cellular organelle is the place for cell respiration, cell energy needs of the various activities, mainly through the mitochondrial respiration to provide. Studies have shown that mitochondrial ATP synthesis in addition to, but also extremely important with a wide range of cell functions, including generation of reactive oxygen species superoxide anion, redox regulation of cell redox potential and conduction, regulation of apoptosis and gene expression and growth in biological , development, aging, disease, death, and the study of biological evolution, there are important theoretical and practical significance, and thus the current life sciences constitute a new growth point.
"Measurement of biological oxygen characteristics of computer analysis system," the organization may be tablets, organizations at the end of tissue, cells, mitochondria and other characteristics of wood samples to measure oxygen and analytical processing, which can be obtained within a certain period of time the oxygen consumption, oxygen consumption change rate, the efficiency of oxidative phosphorylation, respiratory control ratio, and results.

2. Composition: mainly by the electromagnetic stirrer system, measurement of oxygen tanks, oxygen electrode, the system-specific circuit, constant temperature heating circuits, micro-computers, printers, system-specific software, etc..

3 . Characteristics: the system of Biophysics, Chinese Academy of Sciences, the military medical, the medical department of Peking University, Shanghai Second Medical University, Third Military Medical University, Shandong College of Life Sciences, O People's Liberation Army Hospital three years of application and other units, we can see the system has the following salient features:
Test items and more powerful; micro-computer automatic data collection, rapid analysis, and the results stored; compared with manual processing, greatly improving the speed and accuracy of the calculation; to graphic files (the original test curve) and text files ( original data) the form of a large number of experimental data and results stored for at any time to find, retrieve or print output; computer interface clarity can be flexible for a variety of experimental projects and select and set parameters; simple; oxygen electrode design, high sensitivity, dark current small, durable; cost-effective than foreign products.

4 . Principle: Polarographic oxygen electrode is based on principle and made, it is a unique electrochemical gas sensing device, the cathode and anode voltage of the role of a certain polarization, the gas permeable membrane and through the spread of oxygen within the electrolyte resulting electrochemical reaction, the reaction produced a very weak current and the partial pressure of oxygen outside the membrane is proportional to, the weak signal amplification, processing, transformation evacuation analysis of micro-computer processing and computing, data and results into the hard disk. Heating and temperature measuring circuit so that the liquid oxygen tank temperature, electromagnetic stirrer can reduce the pool of liquid temperature gradient.

III.Biological oxygen measurement data analysis software

This software includes the organization of film on the oxygen consumption, organize beads and homogenized, cells, mitochondria, such as the four test items, including data collection, analysis, calculation, if necessary, to graphics or data should be archived, the experiment 23 kinds of tests may also media, sampling period, sampling time, dissolution coefficient, medium size, partial pressure of oxygen values, organization, and the weight of set temperature, ADP value selection or settings. Sampling period can be set, but the sampling time for tens of seconds, the sampling period can be set at 100 ms, sampling time of up to hundreds of seconds, the sampling period may be set for 1000 ms, so in 1000 to ensure that sampling points around a reasonable number of sampling points have data redundancy, sampling points less information will be lost. Dissolution coefficient, medium size, partial pressure of oxygen values, body weight, temperature, ADP values can be modified at any time, together with the unit automatically. Set temperature for the system upgrade to prepare for the future, the system temperature can be measured and fed back to the front-end control system in order to specify the temperature constant. Real-time data window can display the current value of the temperature and oxygen partial pressure, or their relative value. The mouse position of the window can display the current mouse coordinates, such as the status of data analysis in the former data will be turned into a window, change the meaning of Department data showed that the mouse the corresponding point of time and oxygen partial pressure value. Choice of paper can turn a good collection of data files or a new key file, new documents can be by date, the day the number of experiments and test projects, such as auto-naming can also be specified further.

The relevant parameters and, following the specified file name, you can start by sampling of sampling, at the same time will automatically record the current settings as the default value of the next sample. Analysis for different test items have different data processing methods and the corresponding formula, please refer to user manual. Graphics output is to curve analysis and calculation results using a standard BMP file format disk, so that such procedures can be dealt with brush and through the printer to print out.

Analysis of the text output is to be calculated and collected data to standard file formats WRI disk so that you can write procedures, etc. and print them out through the printer.

* Equipment warranty for one year, long-term technical services
* Computer, printer-matching